Birth of piglets after OPS vitrification and transfer of compacted morula stage embryos with intact zona pellucida
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چکیده
منابع مشابه
Birth of piglets after OPS vitrification and transfer of compacted morula stage embryos with intact zona pellucida.
In swine, five to six days post-insemination, morulae and blastocysts are collected together after uterine flushing. The purpose of this study was to vitrify zona pellucida-intact morulae with Open Pulled Straw (OPS) technology and obtain piglets after transfer. Morulae (200) were vitrified after a two-step equilibration in ethylene glycol, dimethyl sulfoxide and sucrose in Hepes-buffered TCM19...
متن کاملBirth of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification.
Pig embryos suffer severe sensitivity to hypothermic conditions, which limits their ability to withstand conventional cryopreservation. Research has focused on high lipid content of pig embryos and its role in hypothermic sensitivity, while little research has been conducted on structural damage. Documenting cytoskeletal disruption provides information on embryonic sensitivity and cellular resp...
متن کاملGlucose metabolism of human morula and blastocyst-stage embryos and its relationship to viability after transfer.
The pregnancy rate and implantation rate following blastocyst transfer in the human have been reported to be high; however, it has remained necessary to transfer 2-3 blastocysts to achieve these rates. Morphological criteria are currently used to select blastocysts for transfer and have some limited correlation with ongoing viability. Glucose metabolism of 189 human morula to blastocyst stage e...
متن کاملDevelopment of early mouse and rabbit embryos without zona pellucida.
Criteria for assessing embryo vitality of denuded rabbit and mouse embryos in vitro were: volume of the embryo, number of cells and number of metaphase plates after 24 and 48 h in culture. Mouse embryos grew normally, with and without the zona pellucida, but denuded rabbit embryos showed reduced growth (volume and cell number) and failure to implant in synchronized recipients.
متن کاملRisk of a Brucella Transmission by Porcine Embryos: an in Vitro Study
Introduction The cryoconservation of pig embryos with intact zona pellucida would be a major advantage to control and then insure minimal risk of disease transmission during embryo transfer of genetic material. Moreover, international rules on embryo transport outside of the country origin require that only embryos with an intact zona pellucida may be exported [9]. A vitrification technique is ...
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ژورنال
عنوان ژورنال: Reproduction Nutrition Development
سال: 2001
ISSN: 0926-5287,1297-9708
DOI: 10.1051/rnd:2001129